1402424: SHENTEK® Trypsin-analog ELISA Kit
1402424: SHENTEK® Trypsin-analog ELISA Kit
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Trypsin-analog are a non-animal origin high-purity recombinant fungal enzyme that is obtained from fermentation production of Fusarium oxysporum DSM2672. Trypsin-analog cleaves peptides on the C-terminal side of lysine and arginine residues as standard trypsin solutions do, which can be used to dissociate a variety of adherent cells, including but not limited to HEK293, A549, Normal Human Epidermal Keratinocytes (NHEKs) and Embryonic Stem Cell (ESC).
This kit is based on the solid-phase Enzyme-linked Immunosorbent Assay (ELISA) with a double-antibody sandwich technique to detect residual Trypsin-analog in the samples. A sheep polyclonal antibody specific to Trypsin-analog was employed in the assay to capture any remaining Trypsin-analog in the samples. Both the Calibration Standard (or test sample) and the HRP (Horseradish Peroxidase) labeled anti-Trypsin-analog antibody were simultaneously added to the microtiter plate, which coated with the affinity purified capture antibody and followed by incubation and washing. Then TMB (3,3',5,5' -tetramethylbenzidine) substrate was added into reaction, HRP catalyzed the oxidation of TMB by H2O2 to produce a blue product (maximum absorption peak at 655 nm). Then the stop solution was added to terminate the enzymatic reaction, resulting in a yellow colored product (maximum absorption peak at 450 nm). The absorbance values at 450 nm wavelength were positively correlated with the Trypsin-analog concentration in the Calibration Standard and the samples. The concentration of Trypsin-analog in the samples can be calculated using the dose-response curve.
Trypsin-analog are a non-animal origin high-purity recombinant fungal enzyme that is obtained from fermentation production of Fusarium oxysporum DSM2672. Trypsin-analog cleaves peptides on the C-terminal side of lysine and arginine residues as standard trypsin solutions do, which can be used to dissociate a variety of adherent cells, including but not limited to HEK293, A549, Normal Human Epidermal Keratinocytes (NHEKs) and Embryonic Stem Cell (ESC).
This kit is based on the solid-phase Enzyme-linked Immunosorbent Assay (ELISA) with a double-antibody sandwich technique to detect residual Trypsin-analog in the samples. A sheep polyclonal antibody specific to Trypsin-analog was employed in the assay to capture any remaining Trypsin-analog in the samples. Both the Calibration Standard (or test sample) and the HRP (Horseradish Peroxidase) labeled anti-Trypsin-analog antibody were simultaneously added to the microtiter plate, which coated with the affinity purified capture antibody and followed by incubation and washing. Then TMB (3,3',5,5' -tetramethylbenzidine) substrate was added into reaction, HRP catalyzed the oxidation of TMB by H2O2 to produce a blue product (maximum absorption peak at 655 nm). Then the stop solution was added to terminate the enzymatic reaction, resulting in a yellow colored product (maximum absorption peak at 450 nm). The absorbance values at 450 nm wavelength were positively correlated with the Trypsin-analog concentration in the Calibration Standard and the samples. The concentration of Trypsin-analog in the samples can be calculated using the dose-response curve.
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When can I expect my order to ship? Most orders are filled and shipped within 2-3 business days from the time they are received. Our standard shipping usually take 2-5 days. We also provide express shippping for time-sensitive deliveries. Email info@genelabx.com if you have any requirements.This is an answer
When can I expect my order to ship? Most orders are filled and shipped within 2-3 business days from the time they are received. Our standard shipping usually take 2-5 days. We also provide express shippping for time-sensitive deliveries. Email info@genelabx.com if you have any requirements.