1301301-1: SHENTEK® E.coli (Protein Expression Strains) HCP ELISA Kit (One-step ELISA)
1301301-1: SHENTEK® E.coli (Protein Expression Strains) HCP ELISA Kit (One-step ELISA)
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This kit employs a solid-phase Enzyme-linked Immunosorbent Assay (ELISA) with a double-antibody sandwich technique to detect residual E.coli (Protein expression strains) host cell proteins (HCPs) in the sample. Polyclonal antibody specific to E.coli (Protein expression strains) HCPs was employed in the assay to capture any remaining HCPs in the sample. Both the Calibration standards (or test sample) and the HRP (Horseradish Peroxidase) labeled with anti-E.coli (Protein expression strains) HCP antibody were simultaneously added to the pre-coated microtiter plate, and followed by incubation and washing steps. Then TMB (3,3',5,5'-tetramethylbenzidine) substrate added for reaction, HRP catalyzed the oxidation of TMB by H2O2 to produce a blue product (maximum absorption peak at 655 nm). Afterwards the stop solution is added to terminate the enzymatic reaction, resulting in a yellow color product (maximum absorption peak at 450 nm). The absorbance values at 450 nm wavelength were positively correlated with the HCPs concentration in the Calibration standard and the sample. The concentration of E.coli (Protein expression strains) in the sample can be calculated using the dose-response curve.
This kit employs a solid-phase Enzyme-linked Immunosorbent Assay (ELISA) with a double-antibody sandwich technique to detect residual E.coli (Protein expression strains) host cell proteins (HCPs) in the sample. Polyclonal antibody specific to E.coli (Protein expression strains) HCPs was employed in the assay to capture any remaining HCPs in the sample. Both the Calibration standards (or test sample) and the HRP (Horseradish Peroxidase) labeled with anti-E.coli (Protein expression strains) HCP antibody were simultaneously added to the pre-coated microtiter plate, and followed by incubation and washing steps. Then TMB (3,3',5,5'-tetramethylbenzidine) substrate added for reaction, HRP catalyzed the oxidation of TMB by H2O2 to produce a blue product (maximum absorption peak at 655 nm). Afterwards the stop solution is added to terminate the enzymatic reaction, resulting in a yellow color product (maximum absorption peak at 450 nm). The absorbance values at 450 nm wavelength were positively correlated with the HCPs concentration in the Calibration standard and the sample. The concentration of E.coli (Protein expression strains) in the sample can be calculated using the dose-response curve.
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When can I expect my order to ship? Most orders are filled and shipped within 2-3 business days from the time they are received. Our standard shipping usually take 2-5 days. We also provide express shippping for time-sensitive deliveries. Email info@genelabx.com if you have any requirements.This is an answer
When can I expect my order to ship? Most orders are filled and shipped within 2-3 business days from the time they are received. Our standard shipping usually take 2-5 days. We also provide express shippping for time-sensitive deliveries. Email info@genelabx.com if you have any requirements.