GL1154：Plasmid midi-prep kit (endotoxin-free) 50 prep/kit
GL1154：Plasmid midi-prep kit (endotoxin-free) 50 prep/kit
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This product is suitable for extracting plasmid DNA up to 80 µ g from 5-15ml bacterial culture medium. The whole extraction process is only 30~60 minutes. This kit uses alkaline lysis method for lysis, and achieves purification by specifically binding DNA to silica gel columns under high salt conditions. The purified plasmid endotoxin is less than 1EU/µ g and can be directly used for cell transfection and other experiments.
Kit Contents
|
Cat.No. |
DP122-01 (10 T) |
DP122-02 (50 T) |
DP122-03 （250 T） |
|
RNase Solution |
60 µl |
300 µl |
750 µl |
|
Buffer S1 |
6 ml |
30 ml |
150 ml |
|
Buffer S2 |
6 ml |
30 ml |
150 ml |
|
Buffer S5 |
6 ml |
30 ml |
150 ml |
|
Bufffer ORT |
2 ml |
10 ml |
50 ml |
|
Buffer W1P |
8 ml |
35 ml |
180 ml |
|
Buffer W2P |
5 ml |
15 ml |
2 x 40 ml |
|
Buffer EB |
2 ml |
10 ml |
50 ml |
|
DNA Extraction Mini Column III |
10 |
50 |
250 |
|
2 ml Collection Tube |
10 |
50 |
250 |
Storage and stability
This product can be stored at room temperature (15~25℃) for 12 months except RNase Solution. At low temperatures, if any precipitates form in the Buffer S2, warm at 37℃ to dissolve. RNase Solution Shipping at room temperature and Stored at-20℃after receipt.Before the kit use, the RNase Solution should be centrifuged briefly, and all of the RNase Solution provided by the kit should be added to the Buffer S1 and stored at 2-8℃.
This product is suitable for extracting plasmid DNA up to 80 µ g from 5-15ml bacterial culture medium. The whole extraction process is only 30~60 minutes. This kit uses alkaline lysis method for lysis, and achieves purification by specifically binding DNA to silica gel columns under high salt conditions. The purified plasmid endotoxin is less than 1EU/µ g and can be directly used for cell transfection and other experiments.
Kit Contents
|
Cat.No. |
DP122-01 (10 T) |
DP122-02 (50 T) |
DP122-03 （250 T） |
|
RNase Solution |
60 µl |
300 µl |
750 µl |
|
Buffer S1 |
6 ml |
30 ml |
150 ml |
|
Buffer S2 |
6 ml |
30 ml |
150 ml |
|
Buffer S5 |
6 ml |
30 ml |
150 ml |
|
Bufffer ORT |
2 ml |
10 ml |
50 ml |
|
Buffer W1P |
8 ml |
35 ml |
180 ml |
|
Buffer W2P |
5 ml |
15 ml |
2 x 40 ml |
|
Buffer EB |
2 ml |
10 ml |
50 ml |
|
DNA Extraction Mini Column III |
10 |
50 |
250 |
|
2 ml Collection Tube |
10 |
50 |
250 |
Storage and stability
This product can be stored at room temperature (15~25℃) for 12 months except RNase Solution. At low temperatures, if any precipitates form in the Buffer S2, warm at 37℃ to dissolve. RNase Solution Shipping at room temperature and Stored at-20℃after receipt.Before the kit use, the RNase Solution should be centrifuged briefly, and all of the RNase Solution provided by the kit should be added to the Buffer S1 and stored at 2-8℃.
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When can I expect my order to ship? Most orders are filled and shipped within 2-3 business days from the time they are received. Our standard shipping usually take 2-5 days. In case of a back order, we will immediately notify the customer by email. We also provide express shippping for time-sensitive deliveries. Email info@genelabx.com if you have any requirements.This is an answer
When can I expect my order to ship? Most orders are filled and shipped within 2-3 business days from the time they are received. Our standard shipping usually take 2-5 days. In case of a back order, we will immediately notify the customer by email. We also provide express shippping for time-sensitive deliveries. Email info@genelabx.com if you have any requirements.