GL2001: Coomassie (Bradford) Protein Quantitative A
GL2001: Coomassie (Bradford) Protein Quantitative A
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Product Description/Introduction
Two of the most commonly used methods for quantifying protein concentration are the Bradford method and the BCA method. The principle of the Bradford method is based on the rapid binding of the Coomassie brilliant blue G250 to proteins. The maximum absorption of Coomassie Brilliant Blue G250 is 488 nm in the free state. After binding to protein, the maximum absorption changed to 595 nm. and the light absorption value is proportional to the content of proteins, so it can be used for the quantitative detection of proteins. This method determines protein concentrations independent of the chemicals in the vast majority of samples, which can be as high as 1 mM for β-mercaptoethanol and 5 mM for dithiothreitol; However, affected by slightly higher concentrations of descaling agents, it is necessary to ensure that the SDS content is less than 0.01%, the Triton X-100 content is less than 0.05%, and the Tween-20, Tween-60, Tween-80, etc. content is less than 0.015%. The BCA Protein Quantitative Detection Kit (G2026) manufactured by our company is recommended for samples containing descaling agents.
This kit is easy and fast to operate, with high sensitivity and extremely fast detection speed, and can detect protein concentrations ranging from 25-1000 μg/mL.
Storage and Shipping Conditions
Product shipped at room temperature. Bovine Serum Albumin (BSA) Standard Ampules stored at 4℃, valid for 12 months. The protein standard solution, prepared with BSA Standard Ampules and Standard Ampules Dilution, store at -20°C and used within 6 months.
Product Description/Introduction
Two of the most commonly used methods for quantifying protein concentration are the Bradford method and the BCA method. The principle of the Bradford method is based on the rapid binding of the Coomassie brilliant blue G250 to proteins. The maximum absorption of Coomassie Brilliant Blue G250 is 488 nm in the free state. After binding to protein, the maximum absorption changed to 595 nm. and the light absorption value is proportional to the content of proteins, so it can be used for the quantitative detection of proteins. This method determines protein concentrations independent of the chemicals in the vast majority of samples, which can be as high as 1 mM for β-mercaptoethanol and 5 mM for dithiothreitol; However, affected by slightly higher concentrations of descaling agents, it is necessary to ensure that the SDS content is less than 0.01%, the Triton X-100 content is less than 0.05%, and the Tween-20, Tween-60, Tween-80, etc. content is less than 0.015%. The BCA Protein Quantitative Detection Kit (G2026) manufactured by our company is recommended for samples containing descaling agents.
This kit is easy and fast to operate, with high sensitivity and extremely fast detection speed, and can detect protein concentrations ranging from 25-1000 μg/mL.
Storage and Shipping Conditions
Product shipped at room temperature. Bovine Serum Albumin (BSA) Standard Ampules stored at 4℃, valid for 12 months. The protein standard solution, prepared with BSA Standard Ampules and Standard Ampules Dilution, store at -20°C and used within 6 months.
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When can I expect my order to ship? Most orders are filled and shipped within 2-3 business days from the time they are received. Our standard shipping usually take 2-5 days. In case of a back order, we will immediately notify the customer by email. We also provide express shippping for time-sensitive deliveries. Email info@genelabx.com if you have any requirements.This is an answer
When can I expect my order to ship? Most orders are filled and shipped within 2-3 business days from the time they are received. Our standard shipping usually take 2-5 days. In case of a back order, we will immediately notify the customer by email. We also provide express shippping for time-sensitive deliveries. Email info@genelabx.com if you have any requirements.