GL2026: BCA protein concentration determination kit. 200T
GL2026: BCA protein concentration determination kit. 200T
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Product Description/Introduction
Bradford and BCA methods are the most commonly used methods for quantitative determination of protein concentration. The BCA Protein Quantitative Detection Kit is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. This method combines the well-known reduction of Cu2+ to Cu+ by protein in an alkaline medium (the biuret reaction) with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu+) using a unique reagent containing bicinchoninic acid. The purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cuprous ion. This water-soluble complex exhibits a strong absorbance at 562 nm that is nearly linear with increasing protein concentrations over a broad working range (50–2000 µg/mL).
This method is not affected by chemical substances and high concentration of detergent in most samples, including 5% SDS, 5% Triton X-100 and 5% Tween-20, 60, 80, etc. However, chelating agent and high concentration reducing agent will affect the detection results. So, it is necessary to ensure that there is no EGTA in the sample, the concentration of EDTA is less than 10 mM, and the concentration of DTT and β-mercaptoethanol is less than 1mM. If the sample contains chelating agent or reducing agent, please consider Bradford protein quantitative detection kit (G2001).
Storage and Shipping Conditions
Ship at room temperature; Protein standard (BSA) store at 2-8℃, valid for 12 months. The protein standard solution should be stored at -20℃ and used within 6 months. The remaining reagents are stored at room temperature, valid for 12 months.
Product Description/Introduction
Bradford and BCA methods are the most commonly used methods for quantitative determination of protein concentration. The BCA Protein Quantitative Detection Kit is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. This method combines the well-known reduction of Cu2+ to Cu+ by protein in an alkaline medium (the biuret reaction) with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu+) using a unique reagent containing bicinchoninic acid. The purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cuprous ion. This water-soluble complex exhibits a strong absorbance at 562 nm that is nearly linear with increasing protein concentrations over a broad working range (50–2000 µg/mL).
This method is not affected by chemical substances and high concentration of detergent in most samples, including 5% SDS, 5% Triton X-100 and 5% Tween-20, 60, 80, etc. However, chelating agent and high concentration reducing agent will affect the detection results. So, it is necessary to ensure that there is no EGTA in the sample, the concentration of EDTA is less than 10 mM, and the concentration of DTT and β-mercaptoethanol is less than 1mM. If the sample contains chelating agent or reducing agent, please consider Bradford protein quantitative detection kit (G2001).
Storage and Shipping Conditions
Ship at room temperature; Protein standard (BSA) store at 2-8℃, valid for 12 months. The protein standard solution should be stored at -20℃ and used within 6 months. The remaining reagents are stored at room temperature, valid for 12 months.
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When can I expect my order to ship? Most orders are filled and shipped within 2-3 business days from the time they are received. Our standard shipping usually take 2-5 days. We also provide express shippping for time-sensitive deliveries. Email info@genelabx.com if you have any requirements.This is an answer
When can I expect my order to ship? Most orders are filled and shipped within 2-3 business days from the time they are received. Our standard shipping usually take 2-5 days. We also provide express shippping for time-sensitive deliveries. Email info@genelabx.com if you have any requirements.